premixed 4× xt loading buffer (Bio-Rad)
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Premixed 4× Xt Loading Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 415 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/loading+buffer+4/XT+Sample+Buffer/us12429486-654-1-6
Average 96 stars, based on 415 article reviews
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Concentration Assay:Article Title: ERG is a regulator of dynamic and reversible endothelial plasticity Article Snippet: .. Samples were prepared for loading using 4 × SDS Page:Article Title: Expression and role of SIRT1 and SIRT6 in porcine conceptus and trophoblasts: relevance to early placenta development†. Article Snippet: .. Samples containing equal amounts of total protein (10 μg for phosphoH3-S10, Cyclin A2, Cyclin B1, and PCNA; 15 μg for SIRT1 and SIRT6; 20 μg for Cyclin D1, phospho-CDK1/2/3 and phospho-CDK2) were dissolved in Article Title: VHL synthetic lethality screens uncover CBF-β as a negative regulator of STING Article Snippet: .. Cells were lysed at ~70–80% confluence in six-well plates with an Staining:Article Title: Expression and role of SIRT1 and SIRT6 in porcine conceptus and trophoblasts: relevance to early placenta development†. Article Snippet: .. Samples containing equal amounts of total protein (10 μg for phosphoH3-S10, Cyclin A2, Cyclin B1, and PCNA; 15 μg for SIRT1 and SIRT6; 20 μg for Cyclin D1, phospho-CDK1/2/3 and phospho-CDK2) were dissolved in Article Title: Rab12 is a regulator of mitophagy and mitochondrial homeostasis Article Snippet: .. Protein samples were boiled at 100°C for 5 minutes with dithiothreitol (Bio-Rad, 1610610) and NuPage LDS Sample Buffer (Thermo Fisher Scientific, NP0007) prior to loading into a 4-20% Incubation:Article Title: Development of a PTEN -siRNA activated scaffold to promote axonal regrowth following spinal cord injury Article Snippet: Proteins were separated by electrophoresis and subsequently transferred to a polyvinylidene fluoride or polyvinylidene difluoride (Trans-Blot Turbo Transfer - 0.2 μm PVDF, BioRad) membrane and blocked with 5 % (w/v) fat-free milk in 0.1 % (v/v) tris-buffered saline (TBS)-Tween 20 (TBST) (Sigma-Aldrich, Ireland). .. Thereafter, 15 μg total protein was incubated at 95 °C with 4x Laemmli sample loading buffer (BioRad) before loading into a Article Title: VHL synthetic lethality screens uncover CBF-β as a negative regulator of STING Article Snippet: .. Cells were lysed at ~70–80% confluence in six-well plates with an |